Summaries by Inventor Holy, Timothy

Light Sheet Microscopy with Objective-Coupled Selective Plane Illumination
Holekamp, Terrence ; Holy, Timothy
T-005916

— Technology Description Researchers in Prof. Timothy Holy’s laboratory at Washington University in St. Louis are among the independent inventors of light sheet fluorescence microscopy and the first to use it for large-scale calcium imaging. This patented technology, OC-SPIM (objective-coupled…

PhOTseq and VNO ectopic expression plasmids
Holy, Timothy ; Lee, Donghoon
T-019206

— These plasmids contain mammalian cell expression promoters and can be used for AAV production. Some plasmids encode a calcium reporter (GCaMP) and photoactivatable fluorescent reporter (PAmCherry). Others plasmids encode a calcium reporter (GCaMP) and one of the vomeronasal receptor genes; two gen…